[Sample submission form is available on: AGNEMA_Testing Submission Form]

Plant parasitic nematodes are minute unsegmented worms that live in soil or plant parts that they infect. They are harmful to host plants and reduce the yield and/or damage the quality of crops. These pests have to be detected in the soil or in the plant parts before proper control actions are taken.


How to sample soil?

Sampling techniques should have backing from diagnostic and identification laboratory to obtain quick and reliable nematode identification. Auger samplers are used to obtain soil cores for nematode population studies at different depths. The soil cores should be obtained from top first foot (12 inches) where most of soil inhabiting nematodes live. Some farmers make a second sample by obtaining soil core from as deep as second foot (24 inches) to ascertain that the deeper seated nematodes are also covered.


How many soil sample should be collected?

Obtaining twenty soil cores per five acres will cover four core per acre is a scheme of sampling that is highly recommended. Various patterns to obtain soil samples is depicted below.

nematode sampling patterns

How much soil?

The soil cores are mixed in a bucket and a pint of soil placed in sampling bag (a paper bag lined with plastic) before the samples are shipped to AGNEMA™ diagnostic lab.


How to preserve and ship samples?

The soil samples should be protected from extreme temperature, direct sun light, drying and freezing. Sample bags are made out of paper lined with plastic however plastic zip lock bag can be used as sample bag instead.

There are 4 main ways to send nematode samples to AGNEMA™ Lab:

  • Bringing the samples to the lab location at:

350 Hills St. Ste 103 Richland WA 99354  (8:00 am to 5:00 pm)

  • Using Drop-off Box in the AGNEMA™ address: available 24/7
  • Using express post services like UPS and FedEx to the AGNEMA™ address
  • Taking the nematode samples to the Soil Testing Laboratories in your area and ask them to do so. We collaborate with major soil testing labs in our region and move along to cover all your Nematode Assay services. Including:

Soiltest Farm Consultants, Inc., Moses Lake, WA

Kuo Testing Labs, Inc., Othello, WA


How to label the samples?

Data like host, locality, Clint’s name, and depth of sample should be written on soil bags as well as Testing Submission Form (http://www.agnema.com/sampling/) before shipping to AGNEMA™.  Specialized nematode assay laboratory, AGNEMA™, will use half of the samples for nematode extraction, and will keep the rest of it for future reference(s).


How to get Sample Submission Form?

Sample Submission Form is available on this Page as AGNEMA_Testing Submission Form  (232 KB)

The information on submitter/client/farmer is needed to send back analytical results and communicate. The sample data including sample ID, depth of sampling, host crop, etc. are also essential for sample analysis.


Plant samples:

Some nematodes damage parts of crop plants, and reside inside the damaged parts. To evaluate the intensity of infection, and extent of damages, the farmer must obtain at least 10 samples per crop and submit them to AGNEMA™ Lab for detection of parasitic nematodes and evaluation of extent of damages.

The infected plant parts like potato tubers are sliced, decolorized, stained and prepared for microscopic observing. The infected plant parts like garlic and onion bulbs, alfalfa crown, and fruit tree roots are immersed in water and incubated in plastic bags for at least 24 hours before nematodes are collected, enumerated and identified.


AGNEMA_Testing Submission Form – (PDF – 232 KB)

Please download the AGNEMA_Testing Submission Form and fill in the details, then send it to the laboratory along with the samples.

Download Soil and Plant Sampling Brochure – (PDF – 595 KB)